Sodium alginate/modified gelatin photothermal antibacterial hydrogel with mesoporous polydopamine-loaded α-N-thiosemicarbazone for promoting wound healing.
Source: PubMed, NCBI / U.S. National Library of Medicine
Refractory wounds caused by bacterial infections pose a severe clinical challenge, highlighting the urgent need for wound dressings with both excellent antibacterial activity and advanced tissue regeneration capabilities. Herein, a multifunctional photothermal antibacterial hydrogel (GHUS-mPDA@HL) was fabricated using mesoporous polydopamine-loaded α-N-thiosemicarbazone (mPDA@HL) nanoparticles, ureido-pyrimidinone grafted gelatin (GHU) and sodium alginate as primary components. The hydrogel network was constructed via quadruple hydrogen bonding and an interpenetrating polymer network, rendering it injectable, self-healing, and tissue-adhesive properties. The mPDA@HL nanoparticles combine the antibacterial and antioxidant properties of α-N-heterocyclic thiosemicarbazone compound (HL) with intrinsic photothermal and antibacterial capacity of mesoporous polydopamine (mPDA). Furthermore, under 808 nm near-infrared irradiation, GHUS-mPDA@HL hydrogel exhibits excellent photothermal performance, enabling efficient bacterial eradication, inflammatory mitigation, and accelerated wound healing. The GHUS-mPDA@HL hydrogel achieves synergistic antibacterial effects against both Gram-negative Escherichia coli (99.98%) and Gram-positive Staphylococcus aureus (99.99%) under near-infrared irradiation. Additionally, the hydrogel showed excellent biocompatibility and effective reactive oxygen species scavenging ability. In a full-thickness skin wound mouse model, GHUS-mPDA@HL
Abstract
Refractory wounds caused by bacterial infections pose a severe clinical challenge, highlighting the urgent need for wound dressings with both excellent antibacterial activity and advanced tissue regeneration capabilities. Herein, a multifunctional photothermal antibacterial hydrogel (GHUS-mPDA@HL) was fabricated using mesoporous polydopamine-loaded α-N-thiosemicarbazone (mPDA@HL) nanoparticles, ureido-pyrimidinone grafted gelatin (GHU) and sodium alginate as primary components. The hydrogel network was constructed via quadruple hydrogen bonding and an interpenetrating polymer network, rendering it injectable, self-healing, and tissue-adhesive properties. The mPDA@HL nanoparticles combine the antibacterial and antioxidant properties of α-N-heterocyclic thiosemicarbazone compound (HL) with intrinsic photothermal and antibacterial capacity of mesoporous polydopamine (mPDA). Furthermore, under 808 nm near-infrared irradiation, GHUS-mPDA@HL hydrogel exhibits excellent photothermal performance, enabling efficient bacterial eradication, inflammatory mitigation, and accelerated wound healing. The GHUS-mPDA@HL hydrogel achieves synergistic antibacterial effects against both Gram-negative Escherichia coli (99.98%) and Gram-positive Staphylococcus aureus (99.99%) under near-infrared irradiation. Additionally, the hydrogel showed excellent biocompatibility and effective reactive oxygen species scavenging ability. In a full-thickness skin wound mouse model, GHUS-mPDA@HL hydrogel under NIR irradiation remarkably accelerates wound repair, with a healing rate of 98.49% within 10 days. It also promotes collagen deposition and angiogenesis to further facilitate wound closure. Overall, this GHUS-mPDA@HL hydrogel presents an excellent antibacterial activity and potent wound-healing promotion effects, providing a promising strategy for the development of intelligent wound dressings.