Prevalence ofand characteristics of gut microbiomes of the infected pigs in Shaanxi province, China.
Source: PubMed, NCBI / U.S. National Library of Medicine
is an obligately intracellular enteric bacterium that infects intestinal epithelial cells and causes porcine proliferative enteropathy (PPE). This study aimed to investigate the epidemiological prevalence ofin large-scale pig farms in Shaanxi Province, China, and analyze the differences in fecal microbial communities of growing pigs with naturalinfection. A total of 672 fecal samples and 300 serum samples were collected from five intensive pig farms in Shaanxi during 2022-2023. Quantitative PCR (qPCR) was used to detectin feces and quantify its fecal load, while enzyme-linked immunosorbent assay (ELISA) was employed to detect the seroprevalence of anti-antibodies in serum. 16S rRNA gene sequencing was performed on fecal samples from-positive and -negative growing pigs. The results showed an overall fecal positive rate of 15.3% (95% CI: 12.7-18.3%) and a serum seroprevalence of 14.7% (95% CI: 11.0-19.3%) forin the surveyed farms, with growing pigs exhibiting the significantly highest positive rate and fecal pathogen load ( < 0.05). Tongchuan exhibited a significantly lower fecal positive rate than the other surveyed regions ( < 0.05). Fecal microbial diversity analysis revealed that-positive fecal samples exhibited significantly higher bacterial species richness. LEfSe analysis indicated a significant enrichment ofin-positive feces relative to negative samples. Network analysis demonstrated a positive correlation betweenand, and random forest analys
Abstract
is an obligately intracellular enteric bacterium that infects intestinal epithelial cells and causes porcine proliferative enteropathy (PPE). This study aimed to investigate the epidemiological prevalence ofin large-scale pig farms in Shaanxi Province, China, and analyze the differences in fecal microbial communities of growing pigs with naturalinfection. A total of 672 fecal samples and 300 serum samples were collected from five intensive pig farms in Shaanxi during 2022-2023. Quantitative PCR (qPCR) was used to detectin feces and quantify its fecal load, while enzyme-linked immunosorbent assay (ELISA) was employed to detect the seroprevalence of anti-antibodies in serum. 16S rRNA gene sequencing was performed on fecal samples from-positive and -negative growing pigs. The results showed an overall fecal positive rate of 15.3% (95% CI: 12.7-18.3%) and a serum seroprevalence of 14.7% (95% CI: 11.0-19.3%) forin the surveyed farms, with growing pigs exhibiting the significantly highest positive rate and fecal pathogen load ( < 0.05). Tongchuan exhibited a significantly lower fecal positive rate than the other surveyed regions ( < 0.05). Fecal microbial diversity analysis revealed that-positive fecal samples exhibited significantly higher bacterial species richness. LEfSe analysis indicated a significant enrichment ofin-positive feces relative to negative samples. Network analysis demonstrated a positive correlation betweenand, and random forest analysis identifiedas the critical microbial biomarker forinfection. This study elucidates the epidemiological characteristics ofin Shaanxi and its interaction with the porcine gut microbiome, thereby providing a theoretical basis for the precise prevention and control of PPE, as well as for further investigations into the interactions between the gut microbiota and.