Morphological and complete genome analysis of a novelphage CpP_VarHS, isolated from wastewater.
Source: PubMed, NCBI / U.S. National Library of Medicine
Various systemic and enteric diseases have been attributed to, including healthcare-associated infections and intoxications. Humans and animals are thus exposed to serious health risks from these bacteria. Infections caused by these antibiotic-resistant bacteria are becoming an increasing concern. Bacteriophages (phages), which possess specific lytic activity, offer a viable alternative for the treatment of. In this study, a novel lyticphage designed as CpP_VarHS was isolated from wastewater and targeted the hosttype A ATCC 13124. The phage was then characterized using Transmission Electron Microscope (TEM), molecular sequencing, and bioinformatics tools. Transmission electron microscopy (TEM) analysis revealed that CpP_VarHS possesses a short, non-contractile tail (31.55 ± 2.5 nm in length) and a regular icosahedral head (44.23 ± 2.1 nm in diameter), characteristic of the podovirus morphotype. Whole-genome analysis showed that the CpP_VarHS genome is 17,814 bp long, linear double-stranded DNA with a GC content of 28.30%. Among the 21 identified open reading frames, 12 were functional genes (GenBank accession number PP973485). Whole-genome sequence comparison showed 94.37% and 94.31% nucleotide identity withphage vB_CpeP_HN02 andphage CPS1. No tRNA, bacterial virulence gene, lysogenic gene, or antibiotic resistance gene was identified in the genome of CpP_VarHS. Phylogenetic analysis indicated that phage CpP_VarHS represents a novel species within the genu
Abstract
Various systemic and enteric diseases have been attributed to, including healthcare-associated infections and intoxications. Humans and animals are thus exposed to serious health risks from these bacteria. Infections caused by these antibiotic-resistant bacteria are becoming an increasing concern. Bacteriophages (phages), which possess specific lytic activity, offer a viable alternative for the treatment of. In this study, a novel lyticphage designed as CpP_VarHS was isolated from wastewater and targeted the hosttype A ATCC 13124. The phage was then characterized using Transmission Electron Microscope (TEM), molecular sequencing, and bioinformatics tools. Transmission electron microscopy (TEM) analysis revealed that CpP_VarHS possesses a short, non-contractile tail (31.55 ± 2.5 nm in length) and a regular icosahedral head (44.23 ± 2.1 nm in diameter), characteristic of the podovirus morphotype. Whole-genome analysis showed that the CpP_VarHS genome is 17,814 bp long, linear double-stranded DNA with a GC content of 28.30%. Among the 21 identified open reading frames, 12 were functional genes (GenBank accession number PP973485). Whole-genome sequence comparison showed 94.37% and 94.31% nucleotide identity withphage vB_CpeP_HN02 andphage CPS1. No tRNA, bacterial virulence gene, lysogenic gene, or antibiotic resistance gene was identified in the genome of CpP_VarHS. Phylogenetic analysis indicated that phage CpP_VarHS represents a novel species within the genusof the family. To the best of our knowledge, this is the first report of the isolation of aphage in Türkiye. In conclusion, these findings suggested that phage CpP_VarHS has promising potential for preventing and controlling.