Design, Synthesis, and Biological Evaluation of Novel‐Acyl Sulfonohydrazides Derived From β‐Hydroxy Esters: Anticancer, Antioxidant, and Antimicrobial Activities

Source: PubMed Central Open Access, NCBI / U.S. National Library of Medicine

ABSTRACT In this study, a series of novel‐acyl sulfonohydrazidesderived from β‐hydroxy esters was synthesized, and their structures were confirmed through spectroscopic characterization, including FT‐IR,H NMR,C NMR, and HRMS analyses. The anticancer properties of the synthesized compounds were investigated, along with a complementary study evaluating their antioxidant and antimicrobial properties. The cytotoxicity screening against HCT116 and PANC1 cancer cell lines revealed generally low antiproliferative effects; however, compoundsanddemonstrated relatively higher pro‐apoptotic potential in HCT116 cells compared to the other compounds. In assays evaluating antioxidant capacity, utilizing both CUPRAC and DPPH methods, all synthesized compounds exhibited activity ranging from moderate to good. Notably, compounddemonstrated the strongest antioxidant performance with TEAC = 1.34 and IC= 0.084 ± 0.003 mmol L. Furthermore, among the tested compounds,,, anddisplayed good antibacterial activity againstwith a minimum inhibitory concentration (MIC) of 19.53 μg/mL. Eighteen novel‐acyl sulfonohydrazide derivativeswere designed and synthesized. All compounds were evaluated in vitro for their antibacterial, antifungal, anticancer, and antioxidant activities. Molecular docking studies were also performed. Compoundsandemerged as the most promising anticancer agents, whileshowed the highest antioxidant capacity. graphical