Dance and Dietary Intervention Improves Metabolic Health, Fitness, and Quality of Life With Modest Gut Microbiota Shifts in Breast Cancer Patients With Obesity: A Pilot RCT

Abstract

ABSTRACT Background Post‐treatment weight gain and gut dysbiosis are important concerns in breast cancer patients. However, evidence on the gut microbiota in this population, particularly in relation to physical activity, is limited. cnr270611-sec-0001 Aim Therefore, we compared gut microbiota in breast cancer patients with obesity with healthy controls and non‐cancer controls with obesity and subsequently examined the effects of a combined dance and dietary intervention on gut microbiota, metabolic health, physical fitness, and quality of life. cnr270611-sec-0002 Methods and Results The observational part compared gut microbiota in breast cancer patients with obesity (BCO, BMI 32.43 ± 4.90 kg/m) with non‐cancer controls with obesity (OC, BMI 37.78 ± 6.68 kg/m) and healthy controls (HC, BMI 21.26 ± 1.26 kg/m). A controlled trial was conducted in breast cancer patients with obesity, with an intervention group (INT,= 13) receiving a 12‐week combined dance and dietary intervention and non‐intervention controls (CTRL,= 10). Gut microbiota was assessed using 16S rRNA sequencing, physical fitness was evaluated by an incremental bicycle ergometer test and motor tests, and quality of life was measured using the EORTC QLQ‐C30 and BR23 questionnaires. In the observational study, breast cancer patients showed significant differences in beta diversity and a lower relative abundance of health‐associated bacteria (e.g.,) compared with both controls. In the controlled trial, the intervention led to a significant improvement in body composition, physical fitness (e.g., Vo2max, handgrip strength), and several validated quality‐of‐life domains (e.g., fatigue, body image). A statistically significant difference in beta diversity at the post‐intervention phylum level was observed (= 0.046,= 0.11). Microbiota composition within INT shifted toward, increased health‐associated taxa (spp.) and reduced opportunistic pathogens (). However, a decrease in butyrate‐producing taxa () was also observed. cnr270611-sec-0003 Conclusion Breast cancer patients showed more negative shifts in gut microbiota compared with both controls. In addition, a 12‐week combined dance and dietary intervention improved body composition, physical fitness, quality of life, and was associated with mixed but potentially beneficial changes in select gut microbiota taxa among breast cancer patients with obesity. cnr270611-sec-0004 Trial Registration Clinical trial registration number: cnr270611-sec-0005