Comparative bioaccumulation and biotransformation of selenate, selenite, and selenomethionine in
Source: PubMed Central Open Access, NCBI / U.S. National Library of Medicine
Selenium (Se) is an essential trace element for humans.is an edible mushroom with nutritional value. To develop a selenium biofortification strategy for., the absorption and transformation of selenium from selenate, selenite, and selenomethionine in mycelia were investigated. The half maximal inhibitory concentrations (IC) of selenate, selenite and selenomethionine were 1028 mg L, 68.2 mg L, and 1.5 mg L, respectively. The kinetic fitting results indicated that the absorption rate of selenomethionine is higher than that of selenite and selenate. The absorption of selenium was inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP) and 2,4-dinitrophenol (2,4-DNP), while the addition of sulfur, phosphorus, and methionine reduced the uptake of selenate, selenite, and selenomethionine by 80.6%, 81.9%, and 14.2%, respectively. The mycelium can convert selenite into elemental selenium at 40 mg L. The major selenium species in selenium-enriched mycelium are selenomethionine, selenocysteine, and Se-methylselenocysteine. Our results indicate that selenium biofortification of.mycelium using selenite is the optimal strategy. The selenium-enriched.mycelium could serve as a novel selenium source in human diets. abs0010 Graphical abstract http://www.w3.org/1999/xlink float portrait ga1.jpg undfig1 anchor portrait graphical abs0015 Highlights • Themycelium are more sensitive to SeMet than Se(VI) and Se(IV). u0010 • Themycelium shows a high ability to bioaccumulate selenium. u0015 • Ene
Abstract
Selenium (Se) is an essential trace element for humans.is an edible mushroom with nutritional value. To develop a selenium biofortification strategy for., the absorption and transformation of selenium from selenate, selenite, and selenomethionine in mycelia were investigated. The half maximal inhibitory concentrations (IC) of selenate, selenite and selenomethionine were 1028 mg L, 68.2 mg L, and 1.5 mg L, respectively. The kinetic fitting results indicated that the absorption rate of selenomethionine is higher than that of selenite and selenate. The absorption of selenium was inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP) and 2,4-dinitrophenol (2,4-DNP), while the addition of sulfur, phosphorus, and methionine reduced the uptake of selenate, selenite, and selenomethionine by 80.6%, 81.9%, and 14.2%, respectively. The mycelium can convert selenite into elemental selenium at 40 mg L. The major selenium species in selenium-enriched mycelium are selenomethionine, selenocysteine, and Se-methylselenocysteine. Our results indicate that selenium biofortification of.mycelium using selenite is the optimal strategy. The selenium-enriched.mycelium could serve as a novel selenium source in human diets. abs0010 Graphical abstract http://www.w3.org/1999/xlink float portrait ga1.jpg undfig1 anchor portrait graphical abs0015 Highlights • Themycelium are more sensitive to SeMet than Se(VI) and Se(IV). u0010 • Themycelium shows a high ability to bioaccumulate selenium. u0015 • Energy metabolism and S, P, and Met pathways are involved in selenium absorption. u0020 • SeCys, SeMet, and MeSeCys are the main selenium species in Se-enriched mycelium. u0025 • Se-enrichedmycelium may serve as a novel dietary selenium source. u0030 simple ulist0010 author-highlights abs0020