Characterization of the microbiome and polyphenolic compounds in the medicinal plant
Source: PubMed Central Open Access, NCBI / U.S. National Library of Medicine
(Maxim) is rich in various chemical constituents and is widely used in traditional Zang medicine. Endophytes play a direct or indirect role in the biosynthesis of active compounds and plant growth. However, little is known about the characteristics of endophytes and polyphenolic compounds in the various organs of. In this study, high-throughput sequencing and polyphenol-targeted metabolomics were employed to analyze endophytic community diversity and assembly processes, polyphenolic compound content, and their correlations. The results showed that the endophytic compositions of the leaf and stem organs were similar, and significantly different from that in the root organs; however, the endophytic diversity did not differ significantly across the various organs. Actinobacteriota and Pseudomonadota were the dominant bacterial phyla, Ascomycota and Basidiomycota were the dominant fungal phyla in the various organs, while the dominant endophytic genera were significantly different. The endophytic community assembly was influenced mainly by stochastic processes in the various organs. A total of 75 polyphenolic compounds were identified, and the contents of the polyphenolic compounds in the various organs ofdiffered significantly. The correlation analysis revealed varying degrees of positive and negative correlation between endophytes and polyphenolic compounds. These findings clarify the characteristics of the endophytes and polyphenolic compounds, and lay a theoretical foundation
Abstract
(Maxim) is rich in various chemical constituents and is widely used in traditional Zang medicine. Endophytes play a direct or indirect role in the biosynthesis of active compounds and plant growth. However, little is known about the characteristics of endophytes and polyphenolic compounds in the various organs of. In this study, high-throughput sequencing and polyphenol-targeted metabolomics were employed to analyze endophytic community diversity and assembly processes, polyphenolic compound content, and their correlations. The results showed that the endophytic compositions of the leaf and stem organs were similar, and significantly different from that in the root organs; however, the endophytic diversity did not differ significantly across the various organs. Actinobacteriota and Pseudomonadota were the dominant bacterial phyla, Ascomycota and Basidiomycota were the dominant fungal phyla in the various organs, while the dominant endophytic genera were significantly different. The endophytic community assembly was influenced mainly by stochastic processes in the various organs. A total of 75 polyphenolic compounds were identified, and the contents of the polyphenolic compounds in the various organs ofdiffered significantly. The correlation analysis revealed varying degrees of positive and negative correlation between endophytes and polyphenolic compounds. These findings clarify the characteristics of the endophytes and polyphenolic compounds, and lay a theoretical foundation for the identification and application functional microbiomes in the.