Antimicrobial resistance, genetic diversity and virulence associated factors ofspp. isolated from poultry meat in Algeria.
Source: PubMed, NCBI / U.S. National Library of Medicine
is the most common cause of bacterial food infections worldwide. In Algeria, data regarding the epidemiology, antimicrobial resistance and virulence ofremain limited. This study aimed to investigate the antimicrobial resistance, genotyping and virulence associated mechanism ofisolated from slaughterhouses in Algeria. A total of 133 pooled poultry samples were collected from five slaughterhouses in Algiers.spp. was differentiated and the antimicrobial susceptibility testing against 12 antimicrobial agents was assessed using Kirby-Bauer-Method. TheO gene and mutations in theA gene associated with tetracyclines and fluoroquinolone resistance were investigated, respectively. The potential associated genes were screened. TheA typing for isolatedwas performed using PCR-RFLP. spp. was detected in 28 (21.05%) poultry samples. The prevalence was significantly higher in caecal samples (61.11%) than in neck skin samples (14.78%). Among 28 isolates, 19 (67.86%), 8 (28.57%) and 1 (3.57%) were identified as,and, respectively. High levels of resistance to fluoroquinolones (92.86%), nalidixic acid (96.43%) and tetracycline (85.71%) were observed. Multidrug resistance to three or more classes of antimicrobial agents was ranged between 7.14 and 28.57%. TheO gene was amplified in 20 (83.3%) phenotypic tetracycline resistant isolates while mutations in theA gene associated with fluoroquinolone resistance were detected in 84.62% (22/26) of isolates.spp. was differentiated into 16 genotypes and tw
Abstract
is the most common cause of bacterial food infections worldwide. In Algeria, data regarding the epidemiology, antimicrobial resistance and virulence ofremain limited. This study aimed to investigate the antimicrobial resistance, genotyping and virulence associated mechanism ofisolated from slaughterhouses in Algeria. A total of 133 pooled poultry samples were collected from five slaughterhouses in Algiers.spp. was differentiated and the antimicrobial susceptibility testing against 12 antimicrobial agents was assessed using Kirby-Bauer-Method. TheO gene and mutations in theA gene associated with tetracyclines and fluoroquinolone resistance were investigated, respectively. The potential associated genes were screened. TheA typing for isolatedwas performed using PCR-RFLP. spp. was detected in 28 (21.05%) poultry samples. The prevalence was significantly higher in caecal samples (61.11%) than in neck skin samples (14.78%). Among 28 isolates, 19 (67.86%), 8 (28.57%) and 1 (3.57%) were identified as,and, respectively. High levels of resistance to fluoroquinolones (92.86%), nalidixic acid (96.43%) and tetracycline (85.71%) were observed. Multidrug resistance to three or more classes of antimicrobial agents was ranged between 7.14 and 28.57%. TheO gene was amplified in 20 (83.3%) phenotypic tetracycline resistant isolates while mutations in theA gene associated with fluoroquinolone resistance were detected in 84.62% (22/26) of isolates.spp. was differentiated into 16 genotypes and two isolates from human were belonged to two differentA genotypes. All 19isolates carriedF andA, whileR,J,B andC were detected in 36.84, 36.84, 84.21 and 52.63%, respectively. In turkeys, 22.22% ofand 100% ofharbored all virulence associated genes. These findings emphasize the importance of integrated surveillance ofacross animal and human sectors to mitigate public health risks.